Zebrafish vs Lumpfish LastZ results
Zebrafish (Danio rerio, GRCz11) and Lumpfish (Cyclopterus lumpus, fCycLum1.pri) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 101. Zebrafish was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Zebrafish | Lumpfish |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 258,365 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Zebrafish |
Uncovered: 1,313,151,514 out of 1,373,471,384 |
Uncovered: 9,427,044 out of 42,031,845 |
| Lumpfish |
Uncovered: 521,660,601 out of 572,885,595 |
Uncovered: 10,765,120 out of 36,193,391 |
Block size distribution
| Size range | All 258,365 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,434 |
6.7 kb |
|||
| 10 bp - 100 bp | 38,758 |
2.8 Mb |
11,470 |
847.8 kb |
|
| 100 bp - 1 kb | 213,677 |
53.0 Mb |
56,403 |
17.2 Mb |
|
| 1 kb - 10 kb | 4,481 |
7.3 Mb |
10,894 |
28.8 Mb |
|
| 10 kb - 100 kb | 13 |
650.0 kb |
696 |
14.8 Mb |
|
| 100 kb - 1 Mb | 2 |
220.6 kb |
15 |
2.4 Mb |
|