Human vs Chinese hamster CHOK1GS LastZ results
Human (Homo sapiens, GRCh38) and Chinese hamster CHOK1GS (Cricetulus griseus, CHOK1GS_HDv1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 90. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Chinese hamster CHOK1GS |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 1,609,170 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 2,037,103,025 out of 3,099,750,718 |
Uncovered: 2,410,180 out of 38,571,754 |
| Chinese hamster CHOK1GS |
Uncovered: 1,392,044,692 out of 2,358,167,390 |
Uncovered: 2,174,662 out of 33,368,010 |
Block size distribution
| Size range | All 1,609,170 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 26,571 |
179.9 kb |
|||
| 10 bp - 100 bp | 162,748 |
8.6 Mb |
11,565 |
787.8 kb |
|
| 100 bp - 1 kb | 1,061,868 |
487.9 Mb |
42,667 |
15.8 Mb |
|
| 1 kb - 10 kb | 357,767 |
618.4 Mb |
15,241 |
36.7 Mb |
|
| 10 kb - 100 kb | 216 |
2.7 Mb |
1,064 |
24.7 Mb |
|
| 100 kb - 1 Mb | 188 |
75.0 Mb |
|||
| 1 Mb - 10 Mb | 141 |
515.0 Mb |
|||
| 10 Mb - 100 Mb | 27 |
450.0 Mb |
|||