Human vs Midas cichlid LastZ results
Human (Homo sapiens, GRCh38) and Midas cichlid (Amphilophus citrinellus, Midas_v5) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Midas cichlid |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 252,401 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,064,406,494 out of 3,099,750,718 |
Uncovered: 17,492,088 out of 38,571,754 |
| Midas cichlid |
Uncovered: 811,237,579 out of 844,902,565 |
Uncovered: 11,493,358 out of 34,548,519 |
Block size distribution
| Size range | All 252,401 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,588 |
7.2 kb |
|||
| 10 bp - 100 bp | 51,150 |
3.8 Mb |
15,723 |
1.2 Mb |
|
| 100 bp - 1 kb | 197,358 |
39.5 Mb |
82,607 |
24.9 Mb |
|
| 1 kb - 10 kb | 2,301 |
3.9 Mb |
10,005 |
19.8 Mb |
|
| 10 kb - 100 kb | 4 |
58.9 kb |
81 |
1.2 Mb |
|
| 100 kb - 1 Mb | 2 |
212.2 kb |
|||