Human vs Zebra mbuna LastZ results
Human (Homo sapiens, GRCh38) and Zebra mbuna (Maylandia zebra, M_zebra_UMD2a) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Zebra mbuna |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options |
Statistics over 264,580 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,066,216,775 out of 3,099,750,718 |
Uncovered: 17,679,883 out of 38,571,754 |
| Zebra mbuna |
Uncovered: 923,132,095 out of 957,485,262 |
Uncovered: 17,637,643 out of 42,396,503 |
Block size distribution
| Size range | All 264,580 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,719 |
7.4 kb |
|||
| 10 bp - 100 bp | 54,103 |
4.1 Mb |
16,356 |
1.2 Mb |
|
| 100 bp - 1 kb | 207,088 |
41.1 Mb |
84,932 |
25.5 Mb |
|
| 1 kb - 10 kb | 1,666 |
2.5 Mb |
10,048 |
19.6 Mb |
|
| 10 kb - 100 kb | 4 |
59.6 kb |
79 |
1.1 Mb |
|
| 100 kb - 1 Mb | 2 |
265.0 kb |
2 |
218.2 kb |
|