Japanese medaka HdrR vs Amazon molly LastZ results
Japanese medaka HdrR (Oryzias latipes, ASM223467v1) and Amazon molly (Poecilia formosa, PoeFor_5.1.2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Japanese medaka HdrR was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Japanese medaka HdrR | Amazon molly |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 350,827 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Japanese medaka HdrR |
Uncovered: 582,327,826 out of 734,057,086 |
Uncovered: 5,790,794 out of 40,334,262 |
| Amazon molly |
Uncovered: 597,140,359 out of 748,923,461 |
Uncovered: 7,294,948 out of 40,804,723 |
Block size distribution
| Size range | All 350,827 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,064 |
6.4 kb |
|||
| 10 bp - 100 bp | 26,076 |
1.7 Mb |
3,244 |
232.8 kb |
|
| 100 bp - 1 kb | 290,044 |
111.4 Mb |
16,480 |
5.7 Mb |
|
| 1 kb - 10 kb | 33,600 |
50.9 Mb |
4,234 |
11.2 Mb |
|
| 10 kb - 100 kb | 43 |
690.1 kb |
802 |
26.7 Mb |
|
| 100 kb - 1 Mb | 340 |
98.8 Mb |
|||
| 1 Mb - 10 Mb | 16 |
22.2 Mb |
|||