Zebrafish vs Asian bonytongue LastZ results
Zebrafish (Danio rerio, GRCz11) and Asian bonytongue (Scleropages formosus, fSclFor1.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 99. Zebrafish was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Zebrafish | Asian bonytongue |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 328,570 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Zebrafish |
Uncovered: 1,313,606,460 out of 1,373,471,384 |
Uncovered: 8,823,362 out of 42,031,845 |
| Asian bonytongue |
Uncovered: 721,809,251 out of 784,546,363 |
Uncovered: 9,873,125 out of 44,035,411 |
Block size distribution
| Size range | All 328,570 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 2,301 |
9.8 kb |
|||
| 10 bp - 100 bp | 49,874 |
3.6 Mb |
13,565 |
1.0 Mb |
|
| 100 bp - 1 kb | 270,672 |
65.6 Mb |
75,644 |
23.5 Mb |
|
| 1 kb - 10 kb | 5,715 |
9.2 Mb |
15,139 |
38.9 Mb |
|
| 10 kb - 100 kb | 8 |
121.6 kb |
718 |
13.7 Mb |
|
| 100 kb - 1 Mb | 10 |
1.4 Mb |
|||