Japanese medaka HdrR vs Red-bellied piranha LastZ results
Japanese medaka HdrR (Oryzias latipes, ASM223467v1) and Red-bellied piranha (Pygocentrus nattereri, fPygNat1.pri) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 112. Japanese medaka HdrR was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Japanese medaka HdrR | Red-bellied piranha |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | 10,100,000 |
| Masking options |
Statistics over 235,942 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Japanese medaka HdrR |
Uncovered: 681,020,751 out of 734,057,086 |
Uncovered: 10,762,132 out of 40,334,262 |
| Red-bellied piranha |
Uncovered: 1,174,038,837 out of 1,222,050,449 |
Uncovered: 19,665,557 out of 46,564,832 |
Block size distribution
| Size range | All 235,942 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,427 |
6.7 kb |
|||
| 10 bp - 100 bp | 36,074 |
2.6 Mb |
8,603 |
632.7 kb |
|
| 100 bp - 1 kb | 194,711 |
47.4 Mb |
43,236 |
13.4 Mb |
|
| 1 kb - 10 kb | 3,723 |
5.9 Mb |
9,604 |
26.0 Mb |
|
| 10 kb - 100 kb | 7 |
86.5 kb |
669 |
13.4 Mb |
|
| 100 kb - 1 Mb | 19 |
2.7 Mb |
|||