Human vs Microbat LastZ results
Human (Homo sapiens, GRCh38) and Microbat (Myotis lucifugus, Myoluc2.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 76. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Microbat |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | |
| Masking options | {default_soft_masking => 1} |
Statistics over 1,730,604 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 1,909,658,643 out of 3,099,750,718 |
Uncovered: 3,904,428 out of 38,571,754 |
| Microbat |
Uncovered: 927,295,388 out of 2,034,575,300 |
Uncovered: 2,975,124 out of 30,512,546 |
Block size distribution
| Size range | All 1,730,604 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 40,662 |
264.2 kb |
|||
| 10 bp - 100 bp | 173,685 |
9.7 Mb |
17,976 |
1.2 Mb |
|
| 100 bp - 1 kb | 1,109,850 |
493.3 Mb |
62,256 |
22.6 Mb |
|
| 1 kb - 10 kb | 406,072 |
739.7 Mb |
18,214 |
45.5 Mb |
|
| 10 kb - 100 kb | 335 |
4.1 Mb |
2,061 |
57.8 Mb |
|
| 100 kb - 1 Mb | 698 |
252.6 Mb |
|||
| 1 Mb - 10 Mb | 276 |
743.3 Mb |
|||
| 10 Mb - 100 Mb | 6 |
124.0 Mb |
|||