Human vs Chinese softshell turtle LastZ results
Human (Homo sapiens, GRCh38) and Chinese softshell turtle (Pelodiscus sinensis, PelSin_1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 76. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Chinese softshell turtle |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options | {default_soft_masking => 1} |
Statistics over 327,091 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,000,501,572 out of 3,096,649,726 |
Uncovered: 8,149,893 out of 35,228,305 |
| Chinese softshell turtle |
Uncovered: 2,122,163,785 out of 2,202,483,752 |
Uncovered: 6,786,200 out of 28,389,223 |
Block size distribution
| Size range | All 327,091 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 703 |
3.5 kb |
|||
| 10 bp - 100 bp | 40,993 |
3.0 Mb |
14,957 |
1.1 Mb |
|
| 100 bp - 1 kb | 273,320 |
78.7 Mb |
122,906 |
42.4 Mb |
|
| 1 kb - 10 kb | 12,072 |
18.8 Mb |
17,616 |
39.0 Mb |
|
| 10 kb - 100 kb | 3 |
45.8 kb |
602 |
13.1 Mb |
|
| 100 kb - 1 Mb | 31 |
5.1 Mb |
|||