Human vs Coquerel's sifaka LastZ results
Human (Homo sapiens, GRCh38) and Coquerel's sifaka (Propithecus coquereli, Pcoq_1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 91. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Coquerel's sifaka |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 2,025,030 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 1,398,806,143 out of 3,096,649,726 |
Uncovered: 990,624 out of 35,444,608 |
| Coquerel's sifaka |
Uncovered: 1,217,014,756 out of 2,798,152,141 |
Uncovered: 593,889 out of 29,815,600 |
Block size distribution
| Size range | All 2,025,030 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 70,667 |
479.3 kb |
|||
| 10 bp - 100 bp | 286,765 |
13.8 Mb |
33,018 |
2.2 Mb |
|
| 100 bp - 1 kb | 1,095,494 |
479.7 Mb |
136,933 |
51.4 Mb |
|
| 1 kb - 10 kb | 569,914 |
1.2 Gb |
47,705 |
95.4 Mb |
|
| 10 kb - 100 kb | 2,190 |
28.2 Mb |
1,856 |
47.5 Mb |
|
| 100 kb - 1 Mb | 578 |
229.6 Mb |
|||
| 1 Mb - 10 Mb | 417 |
1.2 Gb |
|||
| 10 Mb - 100 Mb | 8 |
109.2 Mb |
|||