Human vs Sailfin molly LastZ results
Human (Homo sapiens, GRCh38) and Sailfin molly (Poecilia latipinna, P_latipinna-1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Sailfin molly |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 264,805 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,062,281,946 out of 3,096,649,726 |
Uncovered: 15,123,479 out of 35,619,351 |
| Sailfin molly |
Uncovered: 781,716,557 out of 815,144,743 |
Uncovered: 13,018,983 out of 35,799,401 |
Block size distribution
| Size range | All 264,805 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,570 |
6.8 kb |
|||
| 10 bp - 100 bp | 53,754 |
4.1 Mb |
16,267 |
1.2 Mb |
|
| 100 bp - 1 kb | 207,334 |
41.3 Mb |
87,278 |
26.3 Mb |
|
| 1 kb - 10 kb | 2,140 |
4.2 Mb |
10,410 |
20.8 Mb |
|
| 10 kb - 100 kb | 7 |
174.0 kb |
75 |
1.1 Mb |
|
| 100 kb - 1 Mb | 2 |
212.7 kb |
|||