Zebrafish vs Greater amberjack LastZ results
Zebrafish (Danio rerio, GRCz11) and Greater amberjack (Seriola dumerili, Sdu_1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 94. Zebrafish was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Zebrafish | Greater amberjack |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 325,131 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Zebrafish |
Uncovered: 1,309,341,288 out of 1,373,471,384 |
Uncovered: 8,793,701 out of 42,031,845 |
| Greater amberjack |
Uncovered: 615,458,702 out of 677,686,174 |
Uncovered: 7,173,889 out of 38,650,677 |
Block size distribution
| Size range | All 325,131 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 2,238 |
10.4 kb |
|||
| 10 bp - 100 bp | 50,778 |
3.6 Mb |
16,176 |
1.2 Mb |
|
| 100 bp - 1 kb | 266,624 |
65.6 Mb |
76,981 |
23.1 Mb |
|
| 1 kb - 10 kb | 5,484 |
8.8 Mb |
13,761 |
35.0 Mb |
|
| 10 kb - 100 kb | 7 |
98.6 kb |
808 |
16.4 Mb |
|
| 100 kb - 1 Mb | 15 |
2.5 Mb |
|||