Human vs Argentine black and white tegu LastZ results
Human (Homo sapiens, GRCh38) and Argentine black and white tegu (Salvator merianae, HLtupMer3) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 96. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Argentine black and white tegu |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 277,599 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,022,077,910 out of 3,096,649,726 |
Uncovered: 8,587,869 out of 35,604,148 |
| Argentine black and white tegu |
Uncovered: 1,971,324,345 out of 2,026,218,827 |
Uncovered: 8,442,028 out of 32,145,530 |
Block size distribution
| Size range | All 277,599 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 630 |
3.0 kb |
|||
| 10 bp - 100 bp | 40,047 |
3.0 Mb |
15,203 |
1.1 Mb |
|
| 100 bp - 1 kb | 229,453 |
58.1 Mb |
104,677 |
33.4 Mb |
|
| 1 kb - 10 kb | 7,389 |
15.1 Mb |
14,184 |
34.4 Mb |
|
| 10 kb - 100 kb | 80 |
1.2 Mb |
442 |
7.9 Mb |
|
| 100 kb - 1 Mb | 4 |
618.0 kb |
|||