Human vs Channel bull blenny LastZ results
Human (Homo sapiens, GRCh38) and Channel bull blenny (Cottoperca gobio, fCotGob3.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 98. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Channel bull blenny |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 245,417 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,063,492,792 out of 3,096,649,726 |
Uncovered: 15,242,232 out of 35,619,351 |
| Channel bull blenny |
Uncovered: 576,947,469 out of 609,391,784 |
Uncovered: 13,962,521 out of 37,075,790 |
Block size distribution
| Size range | All 245,417 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,722 |
7.7 kb |
|||
| 10 bp - 100 bp | 49,808 |
3.7 Mb |
14,371 |
1.1 Mb |
|
| 100 bp - 1 kb | 192,177 |
38.3 Mb |
77,284 |
23.6 Mb |
|
| 1 kb - 10 kb | 1,704 |
2.6 Mb |
9,416 |
18.5 Mb |
|
| 10 kb - 100 kb | 5 |
110.8 kb |
92 |
1.5 Mb |
|
| 100 kb - 1 Mb | 1 |
100.2 kb |
2 |
271.1 kb |
|