Opossum vs Koala LastZ results
Opossum (Monodelphis domestica, ASM229v1) and Koala (Phascolarctos cinereus, phaCin_unsw_v4.1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 98. Opossum was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Opossum | Koala |
|---|---|---|
| Chunk size | 10,100,000 | 10,100,000 |
| Overlap | 100,000 | 100,000 |
| Group set size | 10,100,000 | 10,100,000 |
| Masking options | {default_soft_masking => 1} | {default_soft_masking => 1} |
Statistics over 2,361,647 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Opossum |
Uncovered: 1,787,768,889 out of 3,600,504,728 |
Uncovered: 3,142,350 out of 35,083,223 |
| Koala |
Uncovered: 1,408,047,031 out of 3,192,581,492 |
Uncovered: 2,317,438 out of 32,338,536 |
Block size distribution
| Size range | All 2,361,647 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 83,932 |
546.5 kb |
|||
| 10 bp - 100 bp | 238,783 |
11.5 Mb |
23,713 |
1.6 Mb |
|
| 100 bp - 1 kb | 1,371,797 |
631.5 Mb |
85,695 |
28.5 Mb |
|
| 1 kb - 10 kb | 666,079 |
1.3 Gb |
16,717 |
39.4 Mb |
|
| 10 kb - 100 kb | 1,051 |
15.4 Mb |
1,027 |
24.7 Mb |
|
| 100 kb - 1 Mb | 5 |
613.4 kb |
254 |
104.9 Mb |
|
| 1 Mb - 10 Mb | 332 |
1.3 Gb |
|||
| 10 Mb - 100 Mb | 34 |
476.5 Mb |
|||