Human vs Blue-ringed sea krait LastZ results
Human (Homo sapiens, GRCh38) and Blue-ringed sea krait (Laticauda laticaudata, latLat_1.0) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 100. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Blue-ringed sea krait |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 278,193 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,035,882,315 out of 3,096,649,726 |
Uncovered: 9,229,339 out of 35,610,692 |
| Blue-ringed sea krait |
Uncovered: 1,508,721,875 out of 1,558,706,106 |
Uncovered: 4,879,906 out of 23,784,146 |
Block size distribution
| Size range | All 278,193 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 891 |
4.2 kb |
|||
| 10 bp - 100 bp | 43,587 |
3.3 Mb |
16,546 |
1.2 Mb |
|
| 100 bp - 1 kb | 228,211 |
55.4 Mb |
106,811 |
33.9 Mb |
|
| 1 kb - 10 kb | 5,498 |
8.5 Mb |
13,566 |
29.0 Mb |
|
| 10 kb - 100 kb | 6 |
120.8 kb |
212 |
3.2 Mb |
|