Chicken vs Tuatara LastZ results
Chicken (Gallus gallus, bGalGal1.mat.broiler.GRCg7b) and Tuatara (Sphenodon punctatus, ASM311381v1) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 107. Chicken was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Chicken | Tuatara |
|---|---|---|
| Chunk size | 10,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 10,100,000 | 10,100,000 |
| Masking options |
Statistics over 362,116 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Chicken |
Uncovered: 896,397,435 out of 1,053,332,251 |
Uncovered: 5,730,414 out of 31,304,036 |
| Tuatara |
Uncovered: 4,114,791,926 out of 4,272,214,985 |
Uncovered: 5,589,177 out of 25,347,208 |
Block size distribution
| Size range | All 362,116 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 649 |
3.8 kb |
|||
| 10 bp - 100 bp | 20,410 |
1.4 Mb |
5,281 |
390.1 kb |
|
| 100 bp - 1 kb | 312,067 |
120.8 Mb |
84,728 |
36.0 Mb |
|
| 1 kb - 10 kb | 28,985 |
44.7 Mb |
23,019 |
54.2 Mb |
|
| 10 kb - 100 kb | 5 |
55.9 kb |
1,596 |
41.0 Mb |
|
| 100 kb - 1 Mb | 153 |
32.7 Mb |
|||
| 1 Mb - 10 Mb | 2 |
2.7 Mb |
|||