Cow vs Chacoan peccary LastZ results

Cow (Bos taurus, ARS-UCD1.3) and Chacoan peccary (Catagonus wagneri, CatWag_v2_BIUU_UCD) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 111. Cow was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.

Configuration parameters

ParameterValue
Gap open penalty (O)400
Gap extend penalty (E)30
HSP threshold (K)3000
Threshold for gapped extension (L)3000
Threshold for alignments between gapped alignment blocks (H)2200
Masking count (M)
Seed and Transition value (T)1
Scoring matrix (Q)
Default:
     A    C    G    T
    91 -114  -31 -123
  -114  100 -125  -31
   -31 -125  100 -114
  -123  -31 -114   91

Chunking parameters

ParameterCowChacoan peccary
Chunk size10,000,00010,100,000
Overlap0100,000
Group set size10,100,00010,100,000
Masking options

Statistics over 2,344,236 alignment blocks

Genome coverage (bp) Coding exon coverage (bp)
Cow

Uncovered: 1,215,232,139 out of 2,711,209,831
Covered: 1,495,977,692 out of 2,711,209,831

Uncovered: 2,512,689 out of 37,648,360
Matches: 31,038,652 out of 37,648,360
Mismatches: 3,871,562 out of 37,648,360
Insertions: 225,457 out of 37,648,360
Identity over aligned base-pairs: 88.3%

Chacoan peccary

Uncovered: 1,196,461,951 out of 2,640,067,814
Covered: 1,443,605,863 out of 2,640,067,814

Uncovered: 714,911 out of 31,494,020
Matches: 27,719,525 out of 31,494,020
Mismatches: 2,945,606 out of 31,494,020
Insertions: 113,978 out of 31,494,020
Identity over aligned base-pairs: 90.1%

Block size distribution

Size range All 2,344,236 alignment blocks Blocks grouped in nets
# blocks Total size (incl. gaps) # nets Total size (incl. gaps)
1 bp - 10 bp
100,883
647.5 kb
10 bp - 100 bp
234,131
11.7 Mb
15,846
1.1 Mb
100 bp - 1 kb
1,520,903
667.8 Mb
51,895
17.9 Mb
1 kb - 10 kb
487,633
876.6 Mb
11,356
28.8 Mb
10 kb - 100 kb
686
9.2 Mb
990
24.2 Mb
100 kb - 1 Mb
251
86.2 Mb
1 Mb - 10 Mb
209
697.8 Mb
10 Mb - 100 Mb
43
710.1 Mb