Human vs Amazon molly LastZ results
Human (Homo sapiens, GRCh38) and Amazon molly (Poecilia formosa, PoeFor_5.1.2) were aligned using the LastZ alignment algorithm (LastZ) in Ensembl release 76. Human was used as the reference species. After running LastZ, the raw LastZ alignment blocks were chained according to their location in both genomes. During the final netting process, the best sub-chain was chosen in each region on the reference species.
Configuration parameters
| Parameter | Value |
|---|---|
| Gap open penalty (O) | 400 |
| Gap extend penalty (E) | 30 |
| HSP threshold (K) | 3000 |
| Threshold for gapped extension (L) | 3000 |
| Threshold for alignments between gapped alignment blocks (H) | 2200 |
| Masking count (M) | |
| Seed and Transition value (T) | 1 |
| Scoring matrix (Q) | Default:
A C G T
91 -114 -31 -123
-114 100 -125 -31
-31 -125 100 -114
-123 -31 -114 91
|
Chunking parameters
| Parameter | Human | Amazon molly |
|---|---|---|
| Chunk size | 30,000,000 | 10,100,000 |
| Overlap | 0 | 100,000 |
| Group set size | 0 | 10,100,000 |
| Masking options |
Statistics over 280,397 alignment blocks
| Genome coverage (bp) | Coding exon coverage (bp) | |
|---|---|---|
| Human |
Uncovered: 3,060,545,810 out of 3,096,649,726 |
Uncovered: 14,826,821 out of 35,619,351 |
| Amazon molly |
Uncovered: 713,320,720 out of 748,923,461 |
Uncovered: 15,431,205 out of 40,804,723 |
Block size distribution
| Size range | All 280,397 alignment blocks | Blocks grouped in nets | |||
|---|---|---|---|---|---|
| # blocks | Total size (incl. gaps) | # nets | Total size (incl. gaps) | ||
| 1 bp - 10 bp | 1,684 |
7.4 kb |
|||
| 10 bp - 100 bp | 57,886 |
4.3 Mb |
18,879 |
1.4 Mb |
|
| 100 bp - 1 kb | 218,092 |
44.0 Mb |
92,228 |
27.9 Mb |
|
| 1 kb - 10 kb | 2,663 |
4.5 Mb |
11,409 |
22.2 Mb |
|
| 10 kb - 100 kb | 71 |
1.1 Mb |
143 |
2.2 Mb |
|
| 100 kb - 1 Mb | 1 |
118.9 kb |
3 |
403.8 kb |
|